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TCG Medium (DSMZ Medium 1009)

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

TCG Medium

(DSMZ Medium 1009)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Casitone ....................................................................................... 5.0g

Glucose ........................................................................................ 4.0g

Tryptone ....................................................................................... 3.0g

Artificial seawater.........................................................................1.0L

pH 7.5 ± 0.2 at 25°C

Artificial Seawater:

Composition per 10.0mL:

Sea salts, Instant Ocean .............................................................. 22.0g

Preparation of Artificial Seawater: Add Instant Ocean sea salts

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to artificial seawater

and bring volume to 1.0L. Mix thoroughly. Distribute to tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Mechercharimyces mesophilus.