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培养基配方

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Sweet E Broth for Anaerobes

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Gelatin........................................................................................... 0.3g

Cellobiose ..................................................................................... 0.1g

Fructose......................................................................................... 0.1g

Glucose ......................................................................................... 0.1g

L-Arabinose................................................................................... 0.1g

Maltose ......................................................................................... 0.1g

Starch ............................................................................................ 0.1g

Agar .......................................................................................... 0.075g

Peptone ....................................................................................... 0.05g

L-Cysteine·HCl·H2O ................................................................... 0.05g

(NH4)2SO4 .................................................................................. 0.05g

Yeast extract................................................................................ 0.05g

Salts solution............................................................................50.0mL

Rumen fluid .............................................................................30.0mL

Resazurin solution .....................................................................0.4mL

Pyruvic acid .............................................................................0.01mL

pH 6.5 ± 0.2 at 25°C

Salts Solution:

Composition per liter:

NaHCO3...................................................................................... 10.0g

NaCl.............................................................................................. 2.0g

K2HPO4......................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

CaCl2, anhydrous.......................................................................... 0.2g

MgSO4·7H2O................................................................................ 0.2g

Preparation of Salts Solution: Add CaCl2 and MgSO4·7H2O to

distilled/deionized water and bring volume to 300.0mL. Mix thorough

ly. Bring volume to 800.0mL with distilled/deionized water. Add re

maining components while stirring. Bring volume to 1.0L with

distilled/deionized water. Mix thoroughly. Store at 4°C.

Resazurin Solution:

Composition per 44.0mL:

Resazurin .................................................................................. 0.011g

Preparation of Resazurin Solution: Add resazurin to distilled/

deionized water and bring volume to 44.0mL. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Gently heat and

bring to boiling under O2-free 97% N2 + 3% H2. Adjust the pH to 6.5

if necessary. Continue boiling until the medium turns yellow. Distrib

ute into tubes or flasks under O2-free 97% N2 + 3% H2. Cap tubes with

rubber stoppers. Place tubes in a press. Autoclave for 15 min at 15 psi

pressure–121°C with fast exhaust.

Use: For the cultivation and maintenance of Clostridium cocleatum

and Clostridium spiroforme.

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Sweet E Broth for Anaerobes