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Sulfobacillus Medium

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1020.0mL:

Solution A ..............................................................................700.0mL

Solution B ..............................................................................300.0mL

Solution C ................................................................................20.0mL

pH 1.9–2.4 at 25°C

Solution A:

Composition per 700.0mL:

(NH4)2SO4..................................................................................... 3.0g

KCl................................................................................................ 0.1g

K2HPO4......................................................................................... 0.5g

MgSO4·7H2O ................................................................................ 0.5g

Ca(NO3)2..................................................................................... 0.01g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to

2.0–2.2 with sulfuric acid. Autoclave for 15 min at 15 psi pressure–

121°C.

Solution B:

Composition per 300.0mL:

FeSO4·7H2O................................................................................ 44.2g

H2SO4, 10N solution ..................................................................1.0mL

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 300.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Solution C:

Composition per 20.0mL:

Yeast extract.................................................................................. 0.2g

Preparation of Solution C: Add yeast extract to distilled/deionized

water and bring volume to 20.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 700.0mL of solu

tion A, 300.0mL of solution B, and 20.0mL of solution C. Aseptically

adjust pH to 1.9–2.4

Use: For the cultivation of Sulfobacillus thermosulfidooxidans.