Ampicillin TY Salt Medium

[所属分类:培养基配方] [发布时间:2019-10-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ampicillin TY Salt Medium
Composition per liter:
NaCl............................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
Ampicillin solution..................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Ampicillin Solution:
Composition per 10.0mL:
Ampicillin................................................................................50.0mg
Preparation of Ampicillin Solution: Add ampicillin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except ampicillin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL of sterile ampicillin solution. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of various antibiotic resistant bacteria, includ-
ing Escherichia coli.