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Sulfate API Broth

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

Sulfate API Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 10.0g

Sodium lactate .............................................................................. 5.2g

Yeast extract.................................................................................. 1.0g

MgSO4·7H2O................................................................................ 0.2g

Ascorbic acid ................................................................................ 0.1g

Fe(NH4)2(SO4)2·6H2O .................................................................. 0.1g

K2HPO4....................................................................................... 0.01g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add the components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly until dissolved.

Distribute into tubes in 9.0mL volumes. Autoclave for 10 min at 15 psi

pressure–121°C.

Use: For the detection, differentiation, and estimation of sulfate

reducing bacteria.