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Sucrose Phosphate Glutamate Transport Medium

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................ 75.0g

Na2HPO4..................................................................................... 1.22g

Glutamic acid.............................................................................. 0.72g

K2HPO4....................................................................................... 0.52g

Bovine serum...........................................................................50.0mL

Antibiotic inhibitor ..................................................................10.0mL

pH 7.4–7.6 at 25°C

Antibiotic Inhibitor:

Composition per 10.0mL:

Vancomycin .................................................................................. 0.1g

Streptomycin............................................................................... 0.05g

Nystatin................................................................................... 25000U

Preparation of Antibiotic Inhibitor: Add components to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except bovine serum

and antibiotic inhibitor, to distilled/deionized water and bring volume

to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust

pH to 7.4–7.6. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile bovine serum and sterile antibiotic

inhibitor. Mix thoroughly. Aseptically distribute into sterile tubes or

flasks.

Use: For the maintenance of Chlamydia species during transport.