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Sucrose Agar

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

Sucrose Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................ 50.0g

Agar ............................................................................................ 20.0g

Peptone........................................................................................ 10.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 5.0g

CaCO3 ........................................................................................... 3.0g

Phenylethyl alcohol ...................................................................... 3.0g

MgSO4·7H2O................................................................................ 0.5g

MnSO4·4H2O................................................................................ 0.5g

Tween™ 80................................................................................... 0.1g

Bromcresol Green.................................................................... 20.0mg

Cycloheximide solution...........................................................10.0mL

pH 6.2 ± 0.2 at 25°C

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide............................................................................ 0.01g

Preparation of Cycloheximide Solution: Add cycloheximide to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add agar to distilled/deionized water and

bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to

boiling. Add remaining components, except cycloheximide solution

and phenylethyl alcohol, and bring volume to 990.0mL with distilled/

deionized water. Adjust pH to 6.2. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile cy

cloheximide solution and 3.0g of phenylethyl alcohol. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Lactobacillus species from

brewery isolates.