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Streptosel™ Broth

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

Streptosel™ Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Glucose ......................................................................................... 5.0g

Papaic digest of soybean meal ...................................................... 5.0g

NaCl .............................................................................................. 4.0g

Sodium citrate ............................................................................... 1.0g

L-Cystine ....................................................................................... 0.2g

Na2SO3 .......................................................................................... 0.2g

NaN3.............................................................................................. 0.2g

Crystal Violet .............................................................................0.2mg

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Caution: Sodium azide is toxic. Azides also react with metals and

disposal must be highly diluted.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 13 psi pressure–118°C.

Use: For the selective isolation and cultivation of streptococci from

specimens containing a mixed flora.