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Streptococcus Selective Medium

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Special peptone ........................................................................... 23.0g

Agar ............................................................................................ 10.0g

NaCl .............................................................................................. 5.0g

Starch ............................................................................................ 1.0g

Horse blood, defibrinated ........................................................50.0mL

Antibiotic inhibitor ..................................................................10.0mL

pH 7.3± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Antibiotic Inhibitor:

Composition per 10.0mL:

Colistin sulfate .........................................................................10.0mg

Oxolinic acid..............................................................................5.0mg

Preparation of Antibiotic Inhibitor: Add components to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except horse blood and

antibiotic inhibitor, to distilled/deionized water and bring volume to

940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile horse blood and sterile antibiotic inhibitor. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of streptococci from clinical speci

mens or foodstuffs.