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Streptococcus Selection HiVeg Agar with Cycloheximide

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

Streptococcus Selection HiVeg Agar

with Cycloheximide

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Plant hydrolysate ........................................................................ 15.0g

Glucose ......................................................................................... 5.0g

Papaic digest of soybean meal...................................................... 5.0g

NaCl.............................................................................................. 4.0g

Sodium citrate............................................................................... 1.0g

NaN3 ............................................................................................. 0.2g

Na2SO3 ......................................................................................... 0.2g

L-Cystine....................................................................................... 0.2g

Crystal Violet................................................................................ 0.2g

Cycloheximide solution...........................................................10.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide............................................................................ 0.01g

Preparation of Cycloheximide Solution: Add cycloheximide to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except cycloheximide

solution, to distilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 50°C. Asptically add 10.0mL cyclo

heximide solution. Mix thoroughly. Pour into sterile Petri dishes or

aseptically distribute into tubes.

Use: For the selective isolation and enumeration of all types of strep

tococci including group A beta hemolytic strains.