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Streptococcus mutans Medium

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Pancreatic digest of casein............................................................ 2.0g

Mannitol........................................................................................ 0.5g

NaCl ............................................................................................ 0.25g

Lactoalbumin .............................................................................. 0.25g

Agar .......................................................................................... 0.075g

L-Cystine ..................................................................................... 0.05g

Sodium thioglycolate .................................................................. 0.05g

Thallium acetate........................................................................ 0.025g

Crystal Violet .............................................................................0.1mg

Bromcresol Purple (0.04% solution) .......................................15.0mL

pH 7.1 ± 0.2 at 25°C

Caution: Thallium salts are toxic.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes in 5.0mL volumes. Autoclave for 15

min at 15 psi pressure–121°C.

Use: For the selective isolation and cultivation of Streptococcus

mutans. Bacteria that turn the medium yellow are presumptive for

Streptococcus mutans.