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Streptococcus lactis Differential HiVeg Agar Base with Potassium Ferricyanide and Citrate

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

Streptococcus lactis Differential HiVeg Agar Base

with Potassium Ferricyanide and Citrate

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Skim milk (nonfat milk) ............................................................. 10.0g

Glucose ......................................................................................... 5.0g

Plant hydrolysate No. 3................................................................. 2.5g

Potassium ferricyanide solution...............................................10.0mL

Citrate solution.........................................................................10.0mL

pH 6.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Citrate Solution:

Composition per 10.0mL:

Ferric citrate................................................................................ 0.25g

Sodium citrate............................................................................. 0.25g

Preparation of Citrate Solution: Add components to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Sterilize

using flowing steam for 30 min.

Potassium Ferricyanide Solution:

Composition per 10.0mL:

K3[Fe(CN)6] ................................................................................. 1.0g

Preparation of Potassium Ferricyanide Solution: Add 1.0g of

K3[Fe(CN)6] to distilled/deionized water and bring volume to 10.0mL.

Mix thoroughly. Sterilize using flowing steam for 30 min.

Preparation of Medium: Add components, except potassium fer

ricyanide and citrate solution, to distilled/deionized water and bring

volume to 980.0L. Mix thoroughly. Gently heat until boiling. Auto

clave for 12 min at 10 psi pressure–115°C. Cool to 50°C. Add 10.0mL

sterile potassium ferricyanide solution and 10.0mL citrate solution.

Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute

into sterile tubes.

Use: For the differentiation of citrate-utilizing lactic streptococci—

Lactobacillus lactis (Streptococcus lactis) subspecies diacetylactis—

from citrate-nonutilizing Lactobacillus lactis (Streptococcus lactis)

and Lactobacillus lactis (Streptococcus lactis) subspecies cremoris.