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Streptococcus Blood Agar, Selective

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein.......................................................... 10.0g

Beef extract ................................................................................... 6.7g

Nucleic acid .................................................................................. 6.0g

NaCl .............................................................................................. 5.0g

Sheep blood, defibrinated ........................................................50.0mL

Maltose solution.......................................................................10.0mL

Antibiotic inhibitor ..................................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Maltose Solution:

Composition per 10.0mL:

Maltose................................................................................. 0.25–5.0g

Preparation of Maltose Solution: Add maltose to distilled/deion

ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril

ize.

Antibiotic Inhibitor:

Composition per 10.0mL:

Polymyxin B sulfate.................................................................... 0.02g

Neomycin sulfate ........................................................................ 0.01g

Preparation of Antibiotic Inhibitor: Add components to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components—except sheep blood,

maltose solution, and antibiotic inhibitor—to distilled/deionized water

and bring volume to 930.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile sheep blood, sterile maltose solution,

and sterile antibiotic inhibitor. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of group A hemolytic Strepto

coccus species from the human respiratory tract.