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Stonebrink’s Medium

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

Stonebrink’s Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 3040.0mL:

Homogenized whole egg ..............................................................2.0L

Mineral salts solution....................................................................1.0L

Malachite Green solution.........................................................40.0mL

Mineral Salts Solution:

Composition per liter:

Na-pyruvate ................................................................................ 12.5g

KH2PO4......................................................................................... 7.0g

Na2HPO4·7H2O............................................................................. 4.0g

Preparation of Mineral Salts Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Malachite Green Solution:

Composition per 100.0mL:

Malachite Green............................................................................ 2.0g

Preparation of Malchite Green Solution: Add Malachite Green

to distilled/deionized water and bring volume to 100.0mL. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Homogenized Whole Egg:

Composition per 2.0L:

Whole eggs ................................................................................ 36–48

Preparation of Homogenized Whole Egg: Use fresh eggs, less than

1 week old. Scrub the shells with soap. Let stand in a soap solution for 30

min. Rinse in running water. Soak eggs in 70% ethanol for 15 min. Break

the eggs into a sterile container. Homogenize by shaking. Filter through

four layers of sterile cheesecloth into a sterile graduated cylinder. Measure

out 2.0L.

Preparation of Medium: Aseptically add 40.0mL sterile Malachite

Green solution to 1.0L of sterile mineral salts solution. Mix thoroughly.

Aseptically add 2.0L of homogenized whole egg. Mix thoroughly. Dis

tribute into sterile screw-capped tubes. Place tubes in a slanted position.

Inspissate at 85°C (moist heat) for 45 min.

Use: For the cultivation of Mycobacterium species. For the isolation of

Mycobacterium bovis.