编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

Standard Agar with Methanol and Yeast Extract

[所属分类:培养基配方] [发布时间:2021-8-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Base solution..........................................................................982.0mL

Methanol..................................................................................10.0mL

Solution B ..................................................................................5.0mL

Solution A..................................................................................1.0mL

Solution C ..................................................................................1.0mL

Solution D..................................................................................1.0mL

pH 4.0–4.5 at 25°C

Base Solution:

Composition per 982.0mL:

Agar ............................................................................................ 20.0g

Yeast extract.................................................................................. 0.5g

Preparation of Base Solution: Add components to distilled/deion

ized water and bring volume to 982.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C.

Methanol:

Composition per 10.0mL:

Methanol..................................................................................10.0mL

Preparation of Methanol: Filter sterilize.

Solution A:

Composition per liter:

K2HPO4..................................................................................... 87.09g

KH2PO4..................................................................................... 68.05g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Solution B:

Composition per liter:

NH4Cl ..................................................................................... 152.28g

Preparation of Solution B: Add NH4Cl to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Solution C:

Composition per liter:

CaCl2·6H2O ................................................................................ 5.47g

Preparation of Solution C: Add CaCl2·6H2O to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Solution D:

Composition per liter:

MgSO4·7H2O.............................................................................. 71.2g

FeSO4·7H2O.................................................................................. 5.0g

MnSO4·4H2O.............................................................................. 0.81g

CuSO4·5H2O............................................................................... 0.79g

ZnSO4·7H2O ............................................................................... 0.44g

Na2MoO4·2H2O .......................................................................... 0.25g

Preparation of Solution D: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 982.0mL of cooled, sterile base solu

tion, aseptically add 10.0mL of sterile methanol, 1.0mL of sterile solu

tion A, 5.0mL of sterile solution B, 1.0mL of sterile solution C, and

1.0mL of sterile solution D. Mix thoroughly. Adjust pH to 4.0–4.5 if

necessary. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Acetobacter methano

licus.

在线客服

Standard Agar with Methanol and Yeast Extract