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Stan 5 Agar

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

Stan 5 Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution B ..............................................................................650.0mL

Solution A..............................................................................350.0mL

Solution A:

Composition per 350.0mL:

CaCl2·2H2O .................................................................................. 1.0g

(NH4)2SO4 .................................................................................... 1.0g

MgSO4·7H2O................................................................................ 1.0g

Trace elements solution .............................................................1.0mL

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 350.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C.

Trace Elements Solution:

Composition per liter:

EDTA............................................................................................ 8.0g

MnCl2·4H2O ................................................................................. 0.1g

CoCl2 .......................................................................................... 0.02g

KBr ............................................................................................. 0.02g

ZnCl2........................................................................................... 0.02g

CuSO4 ......................................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

NaMoO4·2H2O............................................................................ 0.01g

BaCl2.......................................................................................... 5.0mg

LiCl............................................................................................ 5.0mg

SnCl2·2H2O................................................................................ 5.0mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Solution B:

Composition per 650.0mL:

Agar ............................................................................................ 10.0g

K2HPO4......................................................................................... 1.0g

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 650.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C.

Preparation of Medium: Aseptically combine 350.0mL of cooled,

sterile solution A and 650.0mL of cooled, sterile solution B. Mix thor

oughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of myxobacteria.