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培养基配方

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Staley's Maintenance Broth

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone ......................................................................................... 5.0g

Yeast extract.................................................................................. 0.5g

Hutner's basal salts solution.....................................................20.0mL

Vitamin solution.......................................................................10.0mL

Hutner’s Basal Salts Solution:

Composition per liter:

MgSO4·7H2O.............................................................................. 29.7g

Nitrilotriacetic acid ..................................................................... 10.0g

CaCl2·2H2O .............................................................................. 3.335g

FeSO4·7H2O............................................................................. 99.0mg

(NH4)6Mo7O24·4H2O ............................................................... 9.25mg

“Metals 44”..............................................................................50.0mL

“Metals 44”:

Composition per 100.0mL:

ZnSO4·7H2O ............................................................................. 1.095g

FeSO4·7H2O.................................................................................. 0.5g

Sodium EDTA............................................................................. 0.25g

MnSO4·H2O.............................................................................. 0.154g

CuSO4·5H2O ............................................................................39.2mg

Co(NO3)2·6H2O .......................................................................24.8mg

Na2B4O7·10H2O.......................................................................17.7mg

Preparation of “Metals 44”: Add sodium EDTA to distilled/deion

ized water and bring volume to 90.0mL. Mix thoroughly. Add a few drops

of concentrated H2SO4 to retard precipitation of heavy metal ions. Add re

maining components. Mix thoroughly. Bring volume to 100.0mL with dis

tilled/deionized water.

Preparation of Hutner’s Basal Salts Solution: Add nitrilotria

cetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5

with KOH. Add remaining components. Add distilled/deionized water

to 1.0L. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C.

Vitamin Solution:

Composition per liter:

Nicotinamide..............................................................................9.0mg

Calcium DL-pantothenate...........................................................5.0mg

Riboflavin ..................................................................................5.0mg

Thiamine·HCl ............................................................................5.0mg

Biotin .........................................................................................2.0mg

Folic acid....................................................................................2.0mg

Cyanocobalamin ........................................................................0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except Hutner’s basal

salts solution and vitamin solution, to distilled/deionized water and

bring volume to 970.0mL. Mix thoroughly. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 25°C. Aseptically add 20.0 mL of sterile

Hutner’s basal salts solution and 10.0mL of sterile vitamin solution.

Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Gemmata obscuriglobus.

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Staley's Maintenance Broth