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SS Agar, HiVeg (Salmonella Shigella Agar, HiVeg)

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

SS Agar, HiVeg

(Salmonella Shigella Agar, HiVeg)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Plant peptone............................................................................... 11.5g

Lactose ........................................................................................ 10.0g

Sodium citrate ............................................................................. 10.0g

Na2S2O3 ........................................................................................ 8.5g

Plant extract .................................................................................. 5.0g

Synthetic detergent No. I .............................................................. 2.0g

Ferric citrate.................................................................................. 1.0g

Neutral Red ............................................................................... 0.025g

Brilliant Green .........................................................................0.33mg

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Do not autoclave. Cool to 45°–50°C. Pour

into sterile Petri dishes in 20.0mL volumes. Allow the surface of the

plates to dry before inoculation.

Use: For the selective isolation and differentiation of pathogenic

enteric bacilli, especially those belonging to the genus Salmonella.

This medium is not recommended for the primary isolation of Shigella

species. Lactose-fermenting bacteria such as Escherichia coli or Kleb

siella pneumoniae appear as small pink or red colonies. Lactose-non

fermenting bacteria—such as Salmonella species, Proteus species, and

Shigella species—appear as colorless colonies. Production of H2S by

Salmonella species turns the center of the colonies black.