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Spray’s Fermentation Medium

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Neopeptone................................................................................. 10.0g

Pancreatic digest of casein.......................................................... 10.0g

Agar .............................................................................................. 2.0g

Sodium thioglycolate................................................................ 0.025g

Carbohydrate solution............................................................110.0mL

pH 7.4 ± 0.1 at 25°C

Carbohydrate Solution:

Composition per 200.0mL:

Carbohydrate............................................................................... 20.0g

Preparation of Carbohydrate Solution: Add carbohydrate to

distilled/deionized water and bring volume to 200.0mL. Glucose or

glycerol may be used. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except agar and carbo

hydrate solution, to distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Adjust pH to 7.4. Add agar. Gently heat and

bring to boiling. Distribute into tubes in 9.0mL volumes. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 25°C. Immediately prior to

use heat tubes in a boiling water bath for 10 min. Cool to 45°C. Asep

tically add 1.0mL of sterile carbohydrate solution. Mix thoroughly.

Use: For the cultivation and differentiation of Clostridium perfringens

based on carbohydrate fermentation patterns.