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Sporulation Broth

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]
Sporulation Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Polypeptone™............................................................................. 15.0g
Na2HPO4..................................................................................... 11.0g
Starch, soluble............................................................................... 3.0g
Yeast extract.................................................................................. 3.0g
Sodium thioglycolate .................................................................... 1.0g
MgSO4, anhydrous........................................................................ 0.1g
pH 7.8 ± 0.1 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
in 15.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and observation of sporulation of Clostridium
perfringens.
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