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Sporohalobacter lortetii Broth

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl .......................................................................................... 105.0g

L-Glutamic acid............................................................................. 4.0g

Casamino acids ............................................................................. 2.0g

Nutrient broth................................................................................ 2.0g

Yeast extract.................................................................................. 2.0g

KCl.............................................................................................. 0.75g

L-Cysteine ..................................................................................... 0.5g

FeSO4·7H2O.............................................................................. 0.002g

Resazurin ...................................................................................1.0mg

MgCl2·6H2O solution...............................................................40.0mL

CaCl2·2H2O solution................................................................10.0mL

Trace elements solution ...........................................................10.0mL

Vitamin solution.......................................................................10.0mL

pH 6.5 ± 0.2 at 25°C

MgCl2·6H2O Solution:

Composition per 40.0mL:

MgCl2·6H2O................................................................................ 0.01g

Preparation of MgCl2·6H2O Solution: Add MgCl2·6H2O to dis

tilled/deionized water and bring volume to 40.0mL. Mix thoroughly.

Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

CaCl2·2H2O Solution:

Composition per 40.0mL:

CaCl2·2H2O................................................................................. 0.01g

Preparation of CaCl2·2H2O Solution: Add CaCl2·2H2O to dis

tilled/deionized water and bring volume to 40.0mL. Mix thoroughly.

Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O ............................................................................... 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl .............................................................................................. 1.0g

MnSO4·2H2O ............................................................................... 0.5g

CoSO4·7H2O ............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O................................................................................... 0.1g

FeSO4·7H2O.................................................................................. 0.1g

NiCl2·6H2O ............................................................................... 0.025g

KAl(SO4)2·12H2O....................................................................... 0.02g

CuSO4·5H2O ............................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·2H2O ......................................................................... 0.01g

Na2SeO3·5H2O...........................................................................0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with

KOH. Add remaining components. Add distilled/deionized water to

1.0L.

Vitamin Solution:

Composition per liter:

Pyridoxine·HCl ........................................................................10.0mg

Calcium DL-pantothenate...........................................................5.0mg

Lipoic acid .................................................................................5.0mg

Nicotinic acid.............................................................................5.0mg

p-Aminobenzoic acid.................................................................5.0mg

Riboflavin ..................................................................................5.0mg

Thiamine·HCl ............................................................................5.0mg

Biotin .........................................................................................2.0mg

Folic acid ................................................................................... 2.0mg

Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Prepare and dispense medium under

100% N2. Add components, except MgCl2·6H2O and CaCl2·2H2O so

lutions, to distilled/deionized water and bring volume to 950.0mL. Mix

thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pres

sure–121°C. Aseptically add 40.0mL of sterile MgCl2·6H2O solution

and 10.0mL of sterile CaCl2·2H2O solution. Mix thoroughly. Asepti

cally and anaerobically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Sporohalobacter lortetii.

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Sporohalobacter lortetii Broth