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Spiroplasma Agar MID

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 291.2mL:

Schneider’s Drosophila medium ...........................................160.0mL

Solution 1.................................................................................80.0mL

Fetal calf serum........................................................................50.0mL

Phenol Red (0.5% solution).......................................................1.2mL

pH 7.4 ± 0.2 at 25°C

Schneider’s Drosophila Medium:

Composition per liter:

MgSO4·7H2O................................................................................ 3.7g

NaCl.............................................................................................. 2.1g

Yeast extract.................................................................................. 2.0g

Trehalose....................................................................................... 2.0g

D-Glucose...................................................................................... 2.0g

L-Glutamine .................................................................................. 1.8g

L-Lysine·HCl ................................................................................. 1.7g

L-Proline........................................................................................ 1.7g

KCl................................................................................................ 1.6g

Na2HPO4·7H2O............................................................................. 1.3g

L-Glutamic acid............................................................................. 0.8g

L-Methionine................................................................................. 0.8g

CaCl2, anhydrous.......................................................................... 0.6g

KH2PO4......................................................................................... 0.5g

β-Alanine ...................................................................................... 0.5g

L-Tyrosine ..................................................................................... 0.5g

L-Arginine ..................................................................................... 0.4g

L-Aspartic acid .............................................................................. 0.4g

L-Histidine..................................................................................... 0.4g

L-Threonine................................................................................... 0.4g

NaHCO3........................................................................................ 0.4g

Glycine.......................................................................................... 0.3g

L-Serine ......................................................................................... 0.3g

L-Valine ......................................................................................... 0.3g

L-Isoleucine ................................................................................... 0.2g

L-Leucine....................................................................................... 0.2g

L-Phenylalanine............................................................................. 0.2g

α-Ketoglutaric acid....................................................................... 0.2g

Fumaric acid ................................................................................. 0.1g

Malic acid ..................................................................................... 0.1g

Succinic acid ................................................................................. 0.1g

L-Cystine ....................................................................................... 0.1g

L-Tryptophan ................................................................................. 0.1g

L-Cysteine.................................................................................... 0.06g

Preparation of Schneider’s Drosophila Medium: Add compo

nents to 1.0L of distilled/deionized water. Mix thoroughly. Filter ster

ilize.

Solution 1:

Composition per 80.0mL:

Sorbitol.......................................................................................... 7.0g

Noble agar..................................................................................... 5.0g

Beef heart, solids from infusion.................................................... 5.0g

Peptone.......................................................................................... 1.8g

Sucrose.......................................................................................... 1.0g

Pancreatic digest of casein............................................................ 1.0g

NaCl .............................................................................................. 0.5g

D-Fructose ..................................................................................... 0.1g

D-Glucose ...................................................................................... 0.1g

Preparation of Solution 1: Add components to distilled/deionized

water and bring volume to 80.0mL. Mix thoroughly. Adjust pH to 7.8

with 1N NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°C.

Preparation of Medium: Bring fetal calf serum and Phenol Red so

lution to 56°C. Rapidly bring Schneider’s Drosophila medium to 37°C.

Rapidly combine the components. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Spiroplasma kunkelii and

Spiroplasma species.

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Spiroplasma Agar MID