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Spirolate Broth

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

Spirolate Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Glucose ......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 2.5g

L-Cysteine·HCl·H2O...................................................................... 1.0g

Sodium thioglycolate .................................................................... 0.5g

Palmitic acid ............................................................................... 0.05g

Stearic acid.................................................................................. 0.05g

Oleic acid .................................................................................... 0.05g

Linoleic acid ............................................................................... 0.05g

Serum .....................................................................................100.0mL

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components, except serum, to dis

tilled/deionized water and bring volume to 900.0mL. Mix thoroughly.

Distribute into screw-capped tubes in 20.0mL volumes. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 2.0mL

of serum to each tube. Heat-inactivated sheep, rabbit, or bovine serum

may be used. Tighten caps. Mix thoroughly.

Use: For the cultivation of Treponema phagedenis and other spiro

chetes.