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Spirochete Medium (ATCC Medium 1712)

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

Spirochete Medium

(ATCC Medium 1712)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tris(hydroxymethyl)aminomethane buffer................................. 7.52g

Pancreatic digest of casein............................................................ 1.0g

Yeast extract.................................................................................. 1.0g

L-Cysteine·HCl·2H2O ................................................................... 0.5g

Resazurin ...................................................................................1.0mg

Seawater.................................................................................750.0mL

Glucose solution ......................................................................20.0mL

pH 7.2 ± 0.2 at 25°C

Glucose Solution:

Composition per 20.0mL:

Glucose ......................................................................................... 2.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 20.0mL. Mix thoroughly. Filter steril

ize.

Preparation of Medium: Prepare and dispense medium under

100% N2. Add components, except glucose solution, to distilled/deion

ized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH

to 7.5. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Aseptically add sterile glucose solution. Mix thoroughly. Aseptically

distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Spirochaeta litoralis.