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Spirochete Medium (ATCC Medium 164)

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

Spirochete Medium

(ATCC Medium 164)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

KH2PO4......................................................................................... 1.0g

NH4Cl ........................................................................................... 1.0g

Yeast extract.................................................................................. 1.0g

MgSO4 .......................................................................................... 0.5g

CaCl2........................................................................................... 0.04g

FeCl3·6H2O ..............................................................................1.25mg

NaHCO3 solution .....................................................................20.0mL

Glucose solution ......................................................................10.0mL

Na2S·9H2O solution ...................................................................5.0mL

Glucose Solution:

Composition per 100.0mL:

Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

NaHCO3 Solution:

Composition per 100.0mL:

NaHCO3 ........................................................................................ 5.0g

Preparation of NaHCO3 Solution: Add the NaHCO3 to distilled/

deionized water and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Na2S·9H2O Solution:

Composition per 100.0mL:

Na2S·9H2O.................................................................................. 10.0g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components—except NaHCO3 solu

tion, glucose solution, and Na2S·9H2O solution—to distilled/deionized

water and bring volume to 965.0mL. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add 20.0mL of sterile

NaHCO3 solution, 10.0mL of sterile glucose solution, and 5.0mL of

sterile Na2S·9H2O solution. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the cultivation of spirochetes.