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Spirochaeta zuelzerae Medium

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1...............................................................................480.0mL

Solution 2...............................................................................480.0mL

Solution 3.................................................................................20.0mL

Solution 4.................................................................................20.0mL

pH 7.2 ± 0.2 at 25°C

Solution 1:

Composition per 480.0mL:

KH2PO4....................................................................................... 0.75g

L-Cysteine·HCl·H2O ..................................................................... 0.5g

NaH2PO4·H2O............................................................................. 0.25g

Preparation of Solution 1: Add components to distilled/deionized

water and bring volume to 480.0mL. Mix thoroughly. Adjust pH to 7.2

with 5.0% KOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C.

Solution 2:

Composition per 480.0mL:

NH4Cl ........................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 0.5g

Yeast extract.................................................................................. 0.2g

CaCl2........................................................................................... 0.02g

Resazurin ................................................................................... 1.0mg

FeCl3·6H2O solution (0.25g/L)................................................10.0mL

Trace elements solution .............................................................2.0mL

Preparation of Solution 2: Add components to distilled/deionized

water and bring volume to 480.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Trace Elements Solution:

Composition per 100.0mL:

Na2MoO4·2H2O ........................................................................ 0.075g

H3BO3 ....................................................................................... 0.056g

ZnSO4·7H2O ............................................................................. 0.044g

CoCl2·6H2O ................................................................................ 0.02g

CuSO4·5H2O.............................................................................. 2.0mg

MnCl2......................................................................................... 2.0mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly.

Solution 3:

Composition per 20.0mL:

NaHCO3........................................................................................ 1.0g

Preparation of Solution 3: Add NaHCO3 to distilled/deionized

water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize un

der pressure.

Solution 4:

Composition per 20.0mL:

Glucose ......................................................................................... 2.0g

Preparation of Solution 4: Add glucose to distilled/deionized wa

ter and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically add 480.0 mL of sterile solu

tion 1 to 480.0mL of sterile solution 2 under 80% N2 + 20% CO2.

While gassing, add 20.0mL of sterile solution 3 and 20.0mL of sterile

solution 4. Mix thoroughly. Adjust pH to 7.2. Aseptically and anaero

bically distribute into tubes. Cap with rubber stoppers.

Use: For the cultivation and maintenance of Spirochaeta zuelzerae.

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Spirochaeta zuelzerae Medium