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Spirochaeta stenostrepta Medium

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ......................................................................................... 5.0g

Peptone ......................................................................................... 2.0g

Yeast extract.................................................................................. 0.3g

Vitamin B12 .............................................................................. 0.01mg

Salts solution..........................................................................100.0mL

Phosphate solution...................................................................15.0mL

Sulfide solution........................................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Phosphate Solution:

Composition per liter:

KH2PO4....................................................................................... 30.0g

K2HPO4....................................................................................... 70.0g

Preparation of Phosphate Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter

sterilize.

Salts Solution:

Composition per liter:

MgSO4·7H2O................................................................................ 2.0g

CaCl2·2H2O ................................................................................ 0.75g

EDTA............................................................................................ 0.2g

FeSO4·7H2O.................................................................................. 0.1g

Trace elements solution .............................................................5.0mL

Preparation of Salts Solution: Add EDTA to approximately

800.0mL of distilled/deionized water. Gently heat until dissolved. Ad

just pH to 7.0 with 2.5% KOH. Add the remaining components. Mix

thoroughly. Bring volume to 1.0L with distilled/deionized water.

Trace Elements Solution:

Composition per 1800.0mL:

H3BO3 ........................................................................................... 5.5g

MnCl2·4H2O ................................................................................. 3.5g

AlCl3·6H2O................................................................................... 0.5g

CoCl2·6H2O .................................................................................. 0.5g

CuCl2·2H2O .................................................................................. 0.5g

NiCl2·6H2O................................................................................... 0.5g

ZnCl2............................................................................................. 0.5g

KI ................................................................................................ 0.25g

LiCl ............................................................................................. 0.25g

Na2MoO4·2H2O .......................................................................... 0.25g

BaCl2·2H2O................................................................................. 0.15g

SnCl2·2H2O................................................................................. 0.15g

NaVO3......................................................................................... 0.05g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1800.0mL. Mix thor

oughly. Adjust pH to 3–4 with HCl.

Sulfide Solution:

Composition per 100.0mL:

Na2S·9H2O.................................................................................... 2.0g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de

ionized water and bring volume to 100.0mL. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 25°C. Prepare solution freshly.

Preparation of Medium: Add components, except sulfide solution,

to distilled/deionized water and bring volume to 990.0mL. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile

sulfide solution. Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the isolation of Spirochaeta stenostrepta.

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Spirochaeta stenostrepta Medium