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Spirochaeta aurantia Isolation Medium

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone.......................................................................................... 1.0g

Yeast extract.................................................................................. 1.0g

Hay extract.............................................................................500.0mL

pH 6.5 ± 0.2 at 25°C

Hay Extract:

Composition per liter:

Hay, dried...................................................................................... 5.0g

Preparation of Hay Extract: Add hay to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to

boiling. Continue boiling for 10 min. Filter through Whatman #1 filter

paper.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Spirochaeta aurantia.