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Sphaerotilus Agar (DSMZ Medium 51)

[所属分类:培养基配方] [发布时间:2021-8-20] [发布人:网站管理员2] [阅读次数:] [返回]

Sphaerotilus Agar

(DSMZ Medium 51)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Beef extract, Lab Lemco............................................................... 5.0g

pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.

Gently heat and bring to boiling. Distribute into tubes. Autoclave for

15 min at 15 psi pressure–121°C. Cool in a sloping position to form

slants. Cover solid slants with 2mL sterile tap water. Inoculate into the

covering tap water and incubate at 20°C–25°C.

Use: For the cultivation and maintenance of Sphaerotilus natans.