培养基配方

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Specimen Preservative Medium

[所属分类:培养基配方] [发布时间:2021-8-20] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl .............................................................................................. 5.0g

Sodium citrate·2H2O..................................................................... 5.0g

(NH4)2HPO4.................................................................................. 4.0g

KH2PO4......................................................................................... 2.0g

Yeast extract.................................................................................. 1.0g

Sodium deoxycholate.................................................................... 0.5g

MgSO4·7H2O ................................................................................ 0.4g

Glycerol .................................................................................300.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except glycerol, to dis

tilled/deionized water and bring volume to 700.0mL. Mix thoroughly.

Gently heat and bring to boiling. Add 300.0mL of glycerol. Mix thor

oughly. Distribute into tubes or flasks. Autoclave for 10 min at 11 psi

pressure–116°C.

Use: For the preservation of viable microorganisms in stool speci

mens. For the transport of fecal material.