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Special Infusion Agar, HiVeg with Blood

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Plant infusion.............................................................................. 10.0g

Plant peptone No. 3..................................................................... 10.0g

Plant special infusion.................................................................... 7.5g

NaCl .............................................................................................. 5.0g

Na2HPO4 ....................................................................................... 2.5g

Glucose ......................................................................................... 2.0g

Blood, defibrinated ..................................................................50.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components, except blood, to dis

tilled/deionized water and bring volume to 950.0L. Mix thoroughly.

Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Add 50.0mL sterile defibrinated blood. Mix thor

oughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of a variety of fastidious and nonfastidious aer

obic and anaerobic microorganisms, including streptococci, yeasts, and

molds.