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SP 5 Broth

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

SP 5 Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................ 9.0g

Yeast extract.................................................................................. 1.0g

Artificial seawater.........................................................................1.0L

pH 7.2 ± 0.2 at 25°C

Artificial Seawater:

Composition per liter:

NaCl............................................................................................ 24.7g

MgSO4·7H2O................................................................................ 6.3g

MgCl2·6H2O ................................................................................. 4.6g

CaCl2............................................................................................. 1.0g

KCl................................................................................................ 0.7g

NaHCO3........................................................................................ 0.2g

Preparation of Artificial Seawater: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add solid components to 1.0L of artificial

seawater. Mix thoroughly. Gently heat and bring to boiling. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Cytophaga species, Herpeto

siphon species, Saprospira species, and Flexithrix species.