Amino Acid Assay Medium

[所属分类:培养基配方] [发布时间:2019-10-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Amino Acid Assay Medium
Composition per liter:
Glucose .......................................................................................50.0g
Sodium acetate............................................................................40.0g
NH 4 Cl ...........................................................................................6.0g
KH 2 PO 4 .........................................................................................1.2g
K 2 HPO 4 .........................................................................................1.2g
Asparagine....................................................................................0.8g
L- Glutamic acid.............................................................................0.6g
Pyridoxamine·HCl ........................................................................0.6g
Pyridoxal·HCl...............................................................................0.6g
DL- Valine.......................................................................................0.5g
L- Lysine·HCl.................................................................................0.5g
DL- Isoleucine.................................................................................0.5g
DL- Leucine ....................................................................................0.5g
L- Arginine·HCl..............................................................................0.5g
DL- Threonine.................................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.4g
DL- Alanine.....................................................................................0.4g
DL- Phenylalanine...........................................................................0.2g
L- Tyrosine......................................................................................0.2g
Lysine............................................................................................0.2g
L- Aspartic acid ..............................................................................0.2g
DL- Methionine...............................................................................0.2g
L- Proline........................................................................................0.2g
L- Histidine·HCl...........................................................................0.12g
DL- Serine.......................................................................................0.1g
L- Cystine .......................................................................................0.1g
DL- Tryptophan.............................................................................0.08g
MnSO 4 ·7H 2 O..............................................................................0.04g
FeSO 4 ..........................................................................................0.02g
NaCl............................................................................................0.02g
Adenine sulfate...........................................................................0.02g
Guanine·HCl...............................................................................0.02g
Uracil ..........................................................................................0.02g
Xanthine......................................................................................0.02g
Nicotinic acid.............................................................................2.0mg
Pyridoxine·HCl..........................................................................2.0mg
Thiamine·HCl ............................................................................1.0mg
Calcium pantothenate ................................................................1.0mg
Riboflavin ..................................................................................1.0mg
p-Aminobenzoic acid.................................................................0.2mg
Folic acid..................................................................................0.02mg
Biotin ..........................................................................................2.0μg
pH 6.7 ± 0.2 at 25°C
Source: This medium is available, without cystine, lysine, or methio-
nine, as a premixed powder from BD Diagnostic Systems.
Preparation of Medium: Add components to 1.0L of distilled wa-
ter, omitting the specific amino acid to be assayed for in the procedure.
Heat to boiling for 2–3 min. Distribute into tubes. Autoclave for 10 min
at 15 psi pressure–121°C.
Use: For the microbiological assay for amino acids. Pediococcus aci-
dilactici ATCC 8042 and Enterococcus hirae ATCC 8043 are used as
test microorganisms.