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Soybean Agar
[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]
Soybean Agar
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
White soybeans ......................................................................... 100.0g
Agar ............................................................................................ 15.0g
Preparation of Medium: Add soybeans to 1.0L of distilled/deion
ized water. Soak overnight. Autoclave for 60 min at 15 psi pressure–
121°C. Filter through cheesecloth. Measure volume of filtrate. Add
agar to a concentration of 1.5%. Gently heat and bring to boiling. Dis
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Bacillus subtilis and
Pseudomonas syringae.