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Sorbitol MacConkey Agar (MacConkey Agar with Sorbitol) (BAM M139)

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

Sorbitol MacConkey Agar

(MacConkey Agar with Sorbitol)

(BAM M139)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone or gelysate..................................................................... 17.0g

Agar ............................................................................................ 13.5g

Sorbitol ....................................................................................... 10.0g

NaCl.............................................................................................. 5.0g

Proteose peptone No. 3 or polypeptone........................................ 3.0g

Bile salts, purified......................................................................... 1.5g

Neutral Red................................................................................. 0.03g

Crystal Violet............................................................................. 1.0mg

pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of pathogenic Escherichia coli.