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Sorbitol HiVeg Agar with Blood (Sorbitol MacConkey HiVeg Agar)

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

Sorbitol HiVeg Agar with Blood

(Sorbitol MacConkey HiVeg Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone .............................................................................. 17.0g

Agar ............................................................................................ 13.5g

D-Sorbitol.................................................................................... 10.0g

Plant peptone No. 3....................................................................... 3.0g

Synthetic detergent........................................................................ 1.5g

NaCl .............................................................................................. 5.0g

Neutral Red ................................................................................. 0.03g

Crystal Violet .............................................................................1.0mg

Sterile bovine or sheep blood...................................................50.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium, without blood, is available as a premixed pow

der from HiMedia.

Preparation of Medium: Add components, except blood, to dis

tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile bo

vine blood or sheep blood. Mix thoroughly. Pour into sterile Petri

dishes.

Use: For the isolation and identification of a wide variety of patho

genic bacteria.