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Soil Extract Agar

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

Soil Extract Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Glucose ......................................................................................... 1.0g

K2HPO4......................................................................................... 1.0g

Peptone.......................................................................................... 1.0g

Yeast extract.................................................................................. 1.0g

Soil extract.............................................................................400.0mL

Cycloheximide solution...........................................................10.0mL

pH 6.6 ± 0.2 at 25°C

Soil Extract:

Composition per liter:

Garden soil, neutral.................................................................... 1.0Kg

Preparation of Soil Extract: Add garden soil to 1.0L of tap water.

Autoclave for 20 min at 15 psi pressure–121°C. Filter through What

man filter paper. Bring volume to 1.0L with tap water.

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide............................................................................ 0.04g

Preparation of Cycloheximide Solution: Add cycloheximide to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except cycloheximide

solution, to distilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile

cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For the isolation and cultivation of Arthrobacter species.