AMB Medium

[所属分类:培养基配方] [发布时间:2019-10-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AMB Medium
Composition per liter:
Meat infusion..............................................................................25.0g
K 2 HPO 4 .......................................................................................15.0g
Glucose.........................................................................................5.0g
Yeast extract..................................................................................5.0g
Pancreatic digest of casein............................................................4.0g
L -Cysteine.....................................................................................1.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
Starch, soluble...............................................................................1.0g
MgSO 4 ..........................................................................................0.2g
CaCl 2 ...........................................................................................0.01g
pH 6.9 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: F or the cultivation and maintenance of Eubacterium alactolyti-
cum, Eubacterium budayl, Eubacterium moniliforme, and Eubacte-
rium tortuosum.