Alteromonas Medium (LMG Medium 28)

[所属分类:培养基配方] [发布时间:2019-9-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Alteromonas Medium
(LMG Medium 28)
Composition per 1012mL:
Agar base ......................................................................................1.0L
Methanol..................................................................................10.0mL
Solution A..................................................................................1.0mL
Solution B..................................................................................1.0mL
pH 7.0± 0.2 at 25°C
Agar Base:
Composition per liter:
NaCl............................................................................................20.0g
Agar ............................................................................................15.0g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
K 2 HPO 4 .........................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.3g
Preparation of Agar Base: Add components to 1.0L of distilled/de-
ionized water. Mix thoroughly.
Solution A:
Composition per 100.0mL:
MnSO 4 ·2H 2 O..............................................................................76mg
FeSO 4· 7H 2 O................................................................................28mg
CuSO 4 ·5H 2 O...............................................................................25mg
Na 2 MoO 4 ·2H 2 O ..........................................................................24mg
CoCl 2 ·6H 2 O ................................................................................24mg
CaCl 2 ·2H 2 O.................................................................................15mg
ZnSO 4 ·7H 2 O............................................................................0.14mg
Preparation of Solution A: Add components to 100.0mL of dis-
tilled/deionized water. Mix thoroughly.
Solution B:
Composition per 100.0mL:
Vitamin B 12 ................................................................................0.1mg
Preparation of Solution B: Add Vitamin B 12 to 100.0mL of dis-
tilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add 1.0mL Solution A to 1.0L Agar Base.
Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add 1.0mL sterile Solution B and 10.0mL filter-sterilized methanol.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation of Alteromonas spp.