SB/SW Medium

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

SB/SW Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................1.0g
KCl................................................................................................0.5g
MgCl 2 ·6H 2 O..................................................................................0.4g
NH 4 Cl .........................................................................................0.25g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.15g
Resazurin ...................................................................................1.0mg
Sodium crotonate solution.......................................................50.0mL
NaHCO 3 solution.....................................................................20.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
Seven vitamin solution.............................................................10.0mL
Sodium dithionite solution.......................................................10.0mL
Trace elements solution SL-10 ..................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ...........................................................................190.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10: Prepare and dis-
pense under 80% N 2 + 20% CO 2 . Add FeCl 2 ·4H 2 O to 10.0mL of HCl
solution. Mix thoroughly. Add distilled/deionized water and bring vol-
ume to 1.0L. Add remaining components. Mix thoroughly. Sparge with
80% N 2  + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Sodium Crotonate Solution:
Composition per 50.0mL:
Sodium crotonate..........................................................................1.1g
Preparation of Sodium Crotonate Solution:  Add sodium croto-
nate to distilled/deionized water and bring volume to 50.0mL. Mix
thoroughly. Filter sterilize. Sparge with 80% N 2  + 20% CO 2 .
NaHCO 3 Solution:
Composition per 20.0mL:
NaHCO 3 ........................................................................................2.5g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 20.0mL. Mix thoroughly. Filter ster-
ilize. Sparge with 80% N 2  + 20% CO 2 .
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O..................................................................................0.36g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Seven Vitamin Solution:
Composition per liter:
Pyridoxine·HCl.............................................................................0.3g
Thiamine·HCl...............................................................................0.2g
Nicotinic acid................................................................................0.2g
Calcium  DL -pantothenate..............................................................0.1g
Vitamin B 12 ...................................................................................0.1g
p-Aminobenzoic acid...............................................................80.0mg
Biotin.......................................................................................20.0mg
Preparation of Seven Vitamin Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sodium Dithionite Solution:
Composition per 10.0mL:
Sodium dithioninium....................................................................0.2g
Preparation of Sodium Dithionite Solution:  Add sodium dithi-
oninium to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pres-
sure–121°C.
Preparation of Medium: Prepare medium and dispense under 80%
N 2 + 20% CO 2 . Add components, except sodium crotonate solution,
seven vitamin solution, sodium dithionite solution, NaHCO 3 solution,
and Na 2 S·9H 2 O solution, to distilled/deionized water and bring volume
to 910.0mL. Mix thoroughly. Sparge with 80% N 2 + 20% CO 2 . Auto-
clave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobi-
cally add 50.0mL of sterile sodium crotonate solution, 20.0mL of
sterile NaHCO 3 solution, 10.0mL of seven vitamin solution, and
10.0mL of sterile Na 2 S·9H 2 O solution. Mix thoroughly. Aseptically
and anaerobically distribute into sterile tubes or flasks. After inocula-
tion, add 0.1mL of sodium dithionite solution per 10.0mL of medium.
Use:  For the cultivation of Syntrophobacter buswellii.