Sauton’s Fluid Medium Base

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

Sauton’s Fluid Medium Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

L -Asparagine...............................................................................1.33g
Polysorbate 80 ..........................................................................0.833g
Citric acid....................................................................................0.66g
K 2 HPO 4 .....................................................................................0.177g
NaH 2 PO 4 ...................................................................................0.056g
NaCl..........................................................................................0.035g
Ferric ammonium citrate (brown)...........................................0.0167g
Glycerol ...................................................................................20.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add glycerol to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Add remaining compo-
nents. Mix thoroughly. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the enumeration of mycobacteria.