Salt Tolerance Medium

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

Salt Tolerance Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from..........................................................500.0g
NaCl............................................................................................65.0g
Tryptose......................................................................................10.0g
Glucose.........................................................................................1.0g
Indicator solution.......................................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Indicator Solution:
Composition per 100.0mL:
Bromcresol Purple........................................................................1.6g
Ethanol (95% solution)..........................................................100.0mL
Preparation of Indicator Solution: Add Bromcresol Purple to
ethanol. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of salt-tolerant Streptococcus species and
other salt-tolerant Gram-positive cocci. For the differentiation of
Gram-positive cocci based on salt tolerance.