Salt Polymyxin Broth (SPB)

[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]

Salt Polymyxin Broth
(SPB)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................3.0g
Polymyxin B........................................................................250,000U
pH 8.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.8.
Distribute into tubes or flasks. Autoclave for 10 min at 10 psi pressure–
115°C.
Use: For the isolation and cultivation of Vibrio species from foods.
Salt Polymyxin HiVeg Broth Base with Polymyxin
Composition per liter:
NaCl............................................................................................20.0g
Plant hydrolysate.........................................................................10.0g
Yeast extract..................................................................................3.0g
Selective supplement ...............................................................10.0mL
pH 8.8 ± 0.2 at 25°C
Source: This medium, without selective supplement, is available as a
premixed powder from HiMedia.
Selective Supplement:
Composition per 10.0mL:
Polymyxin B sulfate.............................................................100,000U
Preparation of Selective Supplement: Add polymycin B sulfate
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
or flasks. Mix thoroughly. Adjust pH to 8.8. Distribute into tubes or
flasks. Autoclave for 10 min at 10 psi pressure–115°C. Cool to 50°C.
Aseptically add 10.0mL sterile selective supplement. Mix thoroughly.
Pour into sterile Petri dishes or aseptically distribute into tubes.
Use: For the isolation and cultivation of Vibrio species from foods.