Salmonella Chromogenic Agar (OSCM)

[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]

Salmonella Chromogenic Agar
(OSCM)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Chromogenic mix .......................................................................28.0g
Agar............................................................................................12.0g
Special peptone...........................................................................10.0g
Selective supplement solution.................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Selective Supplement Solution:
Composition per 10.0mL:
Cefsulodin................................................................................12.0mg
Novobiocin ................................................................................5.0mg
Preparation of Selective Supplement Solution: Add components
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0mL Mix thoroughly. Gently heat while
stirring and bring to boiling. Do not autoclave. Cool quickly to 50°C.
Mix thoroughly. Pour into sterile Petri dishes.
Use:  For the identification of Salmonella species and differentiation of
Salmonella spp. other organisms in the family Enterobacteriaceae.
This medium combines two chromogens for the detection of Salmo-
nella spp., 5-bromo-6-chloro-3-indolyl caprylate (Magenta-caprylate)
and 5-bromo-4-chloro-3-indolyl-β- D galactopyranoside (X-gal). X-gal
is a substrate for the enzyme β- D -galactosidase. Hydrolysis of the chro-
mogen, Magenta-caprylate, by lactose-negative Salmonella species
results in magenta colonies. The addition of the selective supplement
solution increases the selectivity of the medium. Novobiocin inhibits
Proteus growth and cefsulodin inhibits growth of pseudomonads.