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Salinivibrio costicola Subspecies vallismortis Medium (DSMZ Medium 597)
[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]
Salinivibrio costicola Subspecies vallismortis Medium
(DSMZ Medium 597)
MgSO 4 ·7H 2 O................................................................................9.6g
MgCl 2 ·6H 2 O..................................................................................7.0g
Glucose .........................................................................................5.0g
KCl................................................................................................3.8g
Yeast extract..................................................................................1.0g
CaCl 2 ·2H 2 O...................................................................................0.5g
K 2 HPO 4 ·3H 2 O...............................................................................0.4g
NaHCO 3 solution ...................................................................100.0mL
pH 7.0 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 100.0mL:
NaHCO 3 ........................................................................................3.0g
Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except NaHCO 3 solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 100.0mL NaHCO 3 solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Salinivibrio costicola subsp. vallismortis.
(DSMZ Medium 597)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
NaCl............................................................................................25.0gMgSO 4 ·7H 2 O................................................................................9.6g
MgCl 2 ·6H 2 O..................................................................................7.0g
Glucose .........................................................................................5.0g
KCl................................................................................................3.8g
Yeast extract..................................................................................1.0g
CaCl 2 ·2H 2 O...................................................................................0.5g
K 2 HPO 4 ·3H 2 O...............................................................................0.4g
NaHCO 3 solution ...................................................................100.0mL
pH 7.0 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 100.0mL:
NaHCO 3 ........................................................................................3.0g
Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except NaHCO 3 solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 100.0mL NaHCO 3 solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Salinivibrio costicola subsp. vallismortis.
