Sabouraud Agar with CCG and 3% Sodium Chloride

[所属分类:培养基配方] [发布时间:2021-8-9] [发布人:网站管理员2] [阅读次数:] [返回]

Sabouraud Agar with CCG and 3% Sodium Chloride

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 3031.5mL:

Glucose.....................................................................................120.0g
NaCl............................................................................................90.0g
Agar............................................................................................45.0g
Peptone .......................................................................................30.0g
Chloramphenicol solution........................................................15.0mL
Cycloheximide solution...........................................................15.0mL
Gentamicin solution...................................................................1.5mL
Chloramphenicol Solution:
Composition per 15.0mL:
Chloramphenicol.........................................................................0.15g
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to distilled/deionized water and bring volume to 15.0mL. Mix thor-
oughly. Filter sterilize.
Cycloheximide Solution:
Composition per 15.0mL:
Cycloheximide..............................................................................0.3g
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 15.0mL. Mix thorough-
ly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Gentamicin Solution:
Composition per 10.0mL:
Gentamicin....................................................................................0.4g
Preparation of Gentamicin Solution: Add gentamicin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components—except chloramphen-
icol solution, cycloheximide solution, and gentamicin solution—to dis-
tilled/deionized water and bring volume to 3.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add 15.0mL of sterile
chloramphenicol solution, 15.0mL of sterile cycloheximide solution,
and 1.5mL of sterile gentamicin solution. Mix thoroughly. Aseptically
distribute into sterile tubes. Allow tubes to cool in a slanted position.
Use: For the selective isolation and cultivation of fungi from speci-
mens with a mixed flora.