SA HiVeg Agar Base with Ampicillin

[所属分类:培养基配方] [发布时间:2021-8-9] [发布人:网站管理员2] [阅读次数:] [返回]

SA HiVeg Agar Base with Ampicillin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Plant hydrolysate ........................................................................10.0g
Starch, soluble.............................................................................10.0g
NaCl..............................................................................................5.0g
Phenol Red................................................................................0.025g
Ampicillin solution..................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium, without ampicillin, is available as a premixed
powder from HiMedia.
Ampicillin Solution:
Composition per 10.0mL:
Ampicillin...................................................................................0.01g
Preparation of Ampicillin Solution: Add ampicillin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except ampicillin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°C. Aseptically add sterile ampicil-
lin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use:  For the isolation, cultivation, and differentiation of Aeromonas
hydrophilia from foods based on starch hydrolysis.