SA Agar

[所属分类:培养基配方] [发布时间:2021-8-9] [发布人:网站管理员2] [阅读次数:] [返回]

SA Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.0g
Starch, soluble...............................................................................1.0g
Ampicillin...................................................................................0.01g
Phenol Red................................................................................0.018g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except ampicillin, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add ampicillin. Mix thor-
oughly. Pour into sterile Petri dishes.
Use: For the isolation, cultivation, and differentiation, based on starch
hydrolysis, of Aeromonas hydrophila from foods. After inoculation of
plates and growth of cultures, starch hydrolysis is determined by flood-
ing each plate with 5.0mL of Lugol’s iodine solution.