Rubritalea Medium (DSMZ Medium 1137)

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Rubritalea Medium
(DSMZ Medium 1137)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone ........................................................................................1.5g
Yeast extract .................................................................................1.5g
Glucose ........................................................................................1.5g
Concentrated artificial seawater ...........................................325.0mL
Hutner’s basal salts solution....................................................20.0mL
Bicarbonate solution................................................................10.0mL
Tris-HCl (1M, pH 7.5) ..............................................................5.0mL
pH 7.5 ± 0.2 at 25°C
Hutner’s Basal Salts Solution:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O...............................................................................3.335g
FeSO 4 ·7H 2 O.............................................................................99.0mg
(NH 4 ) 6 MoO 7 O 24 ·4H 2 O ............................................................9.25mg
“Metals 44”..............................................................................50.0mL
“Metals 44”:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.............................................................................1.095g
FeSO 4 ·7H 2 O..................................................................................0.5g
Sodium EDTA.............................................................................0.25g
MnSO 4 ·H2O..............................................................................0.154g
CuSO 4 ·5H 2 O............................................................................39.2mg
Co(NO 3 ) 2 ·6H 2 O .......................................................................24.8mg
Na 2 B 4 O 7 ·10H 2 O.......................................................................17.7mg
Preparation of “Metals 44”: Add sodium EDTA to distilled/de-
ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few
drops of concentrated H 2 SO 4 to retard precipitation of heavy metal
ions. Add remaining components. Mix thoroughly. Bring volume to
100.0mL with distilled/deionized water.
Preparation of Hutner’s Basal Salts Solution:  Add nitrilotria-
cetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5
with KOH. Add remaining components. Add distilled/deionized water
to 1.0L. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C.
Concentrated Artificial Seawater:
Composition per liter:
NaCl..........................................................................................70.43g
Na 2 SO 4 ......................................................................................11.75g
MgCl 2 ·6H 2 O..............................................................................31.86g
CaCl 2 ·2H 2 O.................................................................................4.35g
KCl..............................................................................................1.99g
KBr..............................................................................................0.29g
H 3 BO 3 .........................................................................................0.08g
Preparation of Concentrated Artificial Seawater: Add com-
ponents to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly.
Bicarbonate Solution :
Composition per 10.0mL:
NaHCO 3 ......................................................................................2.88g
Preparation of Bicarbonate Solution:  Add NaHCO 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Adjust
pH to 6.5. Filter sterilize.
Preparation of Medium: Add components, except bicarbonate so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Adjust pH to 7.5. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Aseptically add bicarbonate solution. Mix thoroughly. Adjust pH to
7.5. Aseptically distribute into culture vessels.
Use: For the cultivation of Rubritalea spp.