Rhodospirillum Medium (ATCC Medium 1408)

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodospirillum Medium
(ATCC Medium 1408)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................100.0g
MgCl 2 ·6H 2 O..................................................................................3.5g
Yeast extract..................................................................................1.5g
Peptone..........................................................................................1.5g
Sodium malate ..............................................................................1.4g
KH 2 PO 4 .........................................................................................0.3g
SLA trace elements....................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
SLA Trace Elements:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.8g
H 3 BO 3 ...........................................................................................0.5g
CoCl 2 ·6H 2 O................................................................................0.25g
ZnCl 2 .............................................................................................0.1g
MnCl 2 ·4H 2 O ...............................................................................0.07g
Na 2 MoO 4 ·2H 2 O..........................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.01g
CuCl 2 ·2H 2 O................................................................................0.01g
Na 2 SeO 3 ·5H 2 O............................................................................0.01g
Preparation of SLA Trace Elements: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 2–3.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust medium to fi-
nal pH of 7.0. Sterilize by autoclaving at 121°C for 15 min.
Use:  For the cultivation of Rhodospirillum species.